Vibrations on the nanoscale (at a length scale of one 1000th of the diameter of a hair and below) have been found to be a ubiquitous phenomenon in living organisms. Recently, our team demonstrated that living cells attached onto a soft cantilever induce nanometric scale oscillations (referred to as nanomotion) that stop as soon as the organism dies. Commercially available atomic force microscopes (AFM) or dedicated devices easily detect these oscillations. Nanomotion detection has been applied to numerous biological samples such as proteins, single organelles, and a plethora of living cells such as prokaryotes (bacteria) and eukaryotes (fungal, vegetal and mammalian cells). The most straightforward application of the technique is the ultra-rapid antibiotic sensitivity test (AST). AST can be performed within an hour as compared to long-lasting traditional AST methods, which depend on the replication rate of the bacteria. The test consists in attaching the organism of interest onto an AFM cantilever and monitoring its oscillations as a function of time upon addition of antibiotics in the analysis chamber. It is worth noting that the nanometric scale oscillations do not only reflect the living or death state of the organisms but also its activity.